designing and development of a dna vaccine based on structural proteins of hepatitis c virus
نویسندگان
چکیده
background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: structural proteins of hcv (core, e1, and e2) were isolated and amplified from jfh strain of hcv genotype 2a using pcr method. the pcr products were cloned into pcdna3.1 (+) vector and finally were confirmed by restriction enzyme analysis and sequencing. the eukaryotic expression of the vector was confirmed by rt-pcr. results: recombinant vector containing 2241bp fragment of hcv structural genes was constructed.the desired plasmid was sequenced and corresponded to 100% identity with the submitted sequences in genbank. rt-pcr results indicated that the recombinant plasmid could be expressed efficiently in the eukaryotic expression system. conclusion: successful cloning of structural viral genes in pcdna3.1 (+) vector and their expression in a eukaryotic expression system facilitates the development of new dna vaccines against hcv. a dna vaccine encoding core-e1-e2 antigens was designed. the desired expression vector can be used for further attempts in the development of vaccines.
منابع مشابه
Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in a eukaryot...
متن کاملDesigning and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus.
BACKGROUND Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in a eukaryotic system. METHODS Struct...
متن کاملHepatitis C Virus and Vaccine Development
The prevalence of Hepatitis C virus (HCV) is approximately 3% around the world. This virus causes chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. The effectiveness of interferon-α and ribavirin therapy is about 50% and is associated with significant toxicity and cost. Hence, generating new vaccines or drugs is an obligation. However, there is no vaccine available for clinical u...
متن کاملImmunogenicity Evaluation of a DNA Vaccine Expressing the Hepatitis C Virus Non-Structural Protein 2 Gene in C57BL/6 Mice
Backgrounds: Most of the hepatitis C virus (HCV) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against HCV is of paramount importance. In this study, we aimed to evaluate co-administration of HCV non-Structural Protein 2 and IL-12 DNA vaccines in C57BL/6 mice. Methods: A plasmid encoding full-length HCV NS2 prot...
متن کاملhepatitis c virus and vaccine development
the prevalence of hepatitis c virus (hcv) is approximately 3% around the world. this virus causes chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. the effectiveness of interferon-α and ribavirin therapy is about 50% and is associated with significant toxicity and cost. hence, generating new vaccines or drugs is an obligation. however, there is no vaccine available for clinical u...
متن کاملEnhanced Immune Responses of a Hepatitis C Virus core DNA Vaccine by co-Inoculating Interleukin-12 Expressing Vector in Mice
Background: Hepatitis C (HCV) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. DNA vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. Objectives: In the present study, a DNA vaccine expressing HCV core protein was developed with IL12 as a genetic a...
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عنوان ژورنال:
iranian journal of pathologyجلد ۱۱، شماره ۳، صفحات ۲۲۲-۲۳۰
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